The museum is housed in a former town hall from the 16th century. It is the first Renaissance building in a civic environment in northern Bohemia. The museum's collections reflect the history of the region from its beginnings to the 20th century. The building is currently being renovated, which is why the exhibition is closed. The museum also includes the Karel Hynek Mácha Memorial in Litoměřice. It is located in the Na Vikárce house, where the poet Karel Hynek Mácha lived and died on November 6, 1836. The house is located at 177/5 Máchova St. Visits must be requested at the ticket office in the administrative building of the Litoměřice Museum or by calling +420 775 868 539.